Antiviral Activity of Total Flavonoid Extracts fromSelaginella moellendorffiiHieron against Coxsackie Virus B3In VitroandIn Vivo

摘要

The antiviral activity of total flavonoid extracts fromSelaginella moellendorffiiHieron and its main constituents amentoflavone were investigated against coxsackie virus B3 (CVB3). When added during or after viral infection, the extracts and amentoflavone prevented the cytopathic effect (CPE) of CVB3, as demonstrated in a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay, with a 50% inhibitory concentration (IC50) from19±1.6to41±1.2 μg/mL and25±1.2to52±0.8 μg/mL, respectively. KM mice were used as animal models to test the extracts' activityin vivo. Oral administration of the total flavonoid extracts at 300 mg/kg/day significantly reduced mean viral titers in the heart and kidneys as well as mortality after infection for 15 days. The experimental results demonstrate thatin vitroandin vivothe model mice infected with CVB3 can be effectively treated by the total flavonoid extracts fromSelaginella moellendorffiiHieron.