Acetonic and Methanolic Extracts ofHeterotheca inuloides, and Quercetin, Decrease CCl4-Oxidative Stress in Several Rat Tissues

摘要

The present study was designed to test the hypothesis that the acetonic and methanolic extracts ofH. inuloidesprevent carbon tetrachloride-(CCl4) induced oxidative stress in vital tissues. Pretreatment with bothH. inuloidesextracts or quercetin attenuated the increase in serum activity of alkaline phosphatase (ALP), total bilirubin (BB), creatinine (CRE), and creatine kinase (CK), and impeded the decrease ofγ-globulin (γ-GLOB) and albumin (ALB) observed in CCl4-induced tissue injury. The protective effect was confirmed by histological analysis with hematoxylin-eosin and periodic acid/Schiff's reagent. Level of lipid peroxidation was higher in the organs of rats exposed to CCl4than in those of the animals treated withHeterohtecaextracts or quercetin, and these showed levels similar to the untreated group. Pretreatment of animals with either of the extracts or quercetin also prevented the increase of 4-hydroxynonenal and 3-nitrotyrosine. Pretreatment with the plant extracts or quercetin attenuated CCl4toxic effects on the activity of several antioxidant enzymes. The present results strongly suggest that the chemopreventive effect of the extracts used and quercetin, against CCl4toxicity, is associated with their antioxidant properties and corroborated previous results obtained in liver tissue.