Objective To investigate the effect of quercetin on cigarette smoke induced oxidative stress and Nrf2 expression. Methods Rats were exposed on cigarette smoke for 4 weeks and treated with quercetin intraperitionally. The lung morphological examination was performed with HE staining. Glutathione and malondialdehyde were measured in lung homogenates and Nrf2 expression were determined with western blot. Results Cigarette smoke significantly increased oxidative stress status and Nrf2 expression in rat lungs and quercetin administration could attenuate cigarette smoke induced airway insults, oxidative stress and Nrf2 high expression. Conclusion Quercetin can decrease cigarette smoke induced oxidative stress and Nrf2 high expression in rat lungs.%目的 探讨槲皮素对香烟暴露大鼠肺组织氧化应激和Nrf2表达的影响.方法 将20只雄性SD大鼠随机分为正常对照组,单纯槲皮素对照组,熏烟模型组,熏烟+槲皮素组,后两组大鼠均接受被动吸烟4周,熏烟+槲皮素组在接受熏烟前予槲皮素50mg/kg·d腹腔注射干预.造模结束后留取肺组织,HE染色观察肺部形态学变化,匀浆肺组织检测谷胱甘肽和丙二醛的变化,Western Blot法检测Nrf2的表达.结果 吸烟能明显增强大鼠肺组织的氧化应激状态和上调Nrf2的表达,槲皮素干预能降低香烟m暴露诱导的肺组织氧化应激状态,降低Nrf2在熏烟大鼠肺组织的表达.结论 槲皮素能降低香烟暴露导致大鼠肺组织的高氧化应激状态,下调熏烟诱导的Nrf2高表达.
展开▼
