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首页> 外文期刊>European review for medical and pharmacological sciences. >LncRNA H19 regulates the expression of its target gene HOXA10 in endometrial carcinoma through competing with miR-612
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LncRNA H19 regulates the expression of its target gene HOXA10 in endometrial carcinoma through competing with miR-612

机译:LncRNA H19通过与miR-612竞争来调节其靶基因HOXA10在子宫内膜癌中的表达

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OBJECTIVE: The role of long non-coding RNA (lncRNA) H19 in endometrial carcinoma was studied, and its mechanism was also explored. PATIENTS AND METHODS: Quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR) was used to detect the expression of lncRNA H19, miR-612, and HOXA10 in endometrial carcinoma, and the relationship between lncRNA H19 and survival time was analyzed. The high expression or knockdown of lncRNA H19 in endometrial cancer cells was completed by cell transfection experiments. Cell counting kit-8 (CCK-8) assay was used to detect changes in the viability of endometrial cancer cells. Dual luciferase reporter assay was performed to verify that miR-612 could bind to lncRNA H19 or HOXA10. QRT-PCR and Western Blot assays were used to detect changes in the expression of HOXA10 in endometrial cancer cells before and after overexpression or knockdown of lncRNA H19. RESULTS: The expression of lncRNA H19 and HOXA10 was high in endometrial carcinoma and miR-612 was lowly expressed. The survival curve suggested that lncRNA H19 was negatively correlated with patient survival. The mRNA expression of lncRNA H19 in endometrial cancer cells, including HEC1-A, HEC1-B, AN3CA, and Ishikawa, was detected by qRT-PCR. It was found that the expression of lncRNA H19 was highest in AN3CA and lowest in Ishikawa. The cell transfection experiments allowed Ishikawa cells to overexpress lncRNA H19 and AN3CA cells to reduce lncRNA H19 expression. After overexpression of lncRNA H19, the viability of Ishikawa cells as well as the mRNA and protein levels of HOXA10 increased. However, after knocking down lncRNA H19, the viability of AN3CA cells along with the mRNA and protein levels of HOXA10 decreased. The dual luciferase reporter assay results suggested that miR-612 could bind to lncRNA H19 and HOXA10. CONCLUSIONS: The high expression of lncRNA H19 in endometrial carcinoma may regulate the expression level of its target gene HOXA10 by targeting miR-612, thus promoting cell proliferation to play a role in the development of endometrial carcinoma.
机译:目的:研究长非编码RNA(IncRNA)H19在子宫内膜癌中的作用,并探讨其机制。病人与方法:采用定量逆转录聚合酶链反应(qRT-PCR)检测子宫内膜癌中lncRNA H19,miR-612和HOXA10的表达,并分析lncRNA H19与生存时间的关系。通过细胞转染实验完成了子宫内膜癌细胞中lncRNA H19的高表达或敲低。细胞计数试剂盒8(CCK-8)分析用于检测子宫内膜癌细胞活力的变化。进行双重荧光素酶报告基因测定以验证miR-612可以结合lncRNA H19或HOXA10。使用QRT-PCR和Western Blot分析检测lncRNA H19过表达或敲除前后子宫内膜癌细胞HOXA10表达的变化。结果:子宫内膜癌中lncRNA H19和HOXA10表达高,miR-612表达低。生存曲线表明lncRNA H19与患者生存呈负相关。通过qRT-PCR检测了子宫内膜癌细胞包括HEC1-A,HEC1-B,AN3CA和Ishikawa在内的lncRNA H19的mRNA表达。发现lncRNA H19的表达在AN3CA中最高而在石川中最低。细胞转染实验允许Ishikawa细胞过表达lncRNA H19和AN3CA细胞以降低lncRNA H19表达。 lncRNA H19过表达后,石川细胞的活力以及HOXA10的mRNA和蛋白质水平增加。然而,敲低lncRNA H19后,AN3CA细胞的活力以及HOXA10的mRNA和蛋白水平下降。双重荧光素酶报告基因测定结果表明,miR-612可以与lncRNA H19和HOXA10结合。结论:lncRNA H19在子宫内膜癌中的高表达可能通过靶向miR-612来调节其靶基因HOXA10的表达水平,从而促进细胞增殖在子宫内膜癌的发展中发挥作用。

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