Genistein inhibits expression of matrix metalloproteinases-2 (MMP-2) in premature ovarian failure (POF) rats induced with cisplatin intraperitoneal injection

摘要

Matrix metalloproteinases (MMPs) play a pivotal role in ovary disease by degrading multiple elements of the extracellular matrix (ECM). Therefore, the inhibition of MMPs has been suggested to be a promising therapeutic strategy for premature ovarian failure(POF).?Genistein, the primary isoflavone in legumes, has a well?known?weak estrogenic effect by binding to estrogen receptors, and widely used in the treatment of POF;however, MMPs changes after using genistein?(Gen)?was unclear.?The aim of our study was to evaluate changes from MMP-2?of?POF?rats induced by?cisplatin intraperitoneal injection after using different dose?of GEN treatment by western-blot and immunohistochemistry technology. The results demonstrated that MMP?was significantly down-regulated in middle dose GEN group and high dose of GEN group in comparison to the?cisplatin?group?on protein level?(P < 0.05), and?middle dose GEN group, high dose of GEN group?and control group had no significant difference?on mRNA level?(P < 0.05). The present study provides?improvement in understanding the molecular pathogenic mechanism of POF?and development of Gen as effective treatment drugs.