Staphylococcal cassette chromosome mec (SCCmec) analysis and antimicrobial susceptibility profiles of methicillin-resistant staphylococcus aureus (MRSA) isolates in a teaching hospital, Shantou, China

摘要

Methicillin-resistant?staphylococcus aureus?(MRSA) infection has now become a major public health concern. The aim of this study is to determine the antibiotic resistance pattern and prevalence of different staphylococcal cassette chromosome mec (SCCmec) types among the MRSA isolates from a teaching hospital in Shantou, China. The minimum inhibitory concentrations (MICs) ofseventeen antimicrobial agents against MRSA isolates?were determined using the micro broth dilution method. SCCmec types were identified by multiplexpolymerase chain reaction?(PCR) strategy. The results show that all MRSA isolates were resistant to ampicillin, oxacillin, gentamycin, erythromycin and ciprofloxacin. Fewer than 6% of isolates were resistant to doxycycline, but more than 90% were resistant to cefazolin, cefuroxime, cefotaxime, cefepime, sparfloxacin and tetracycline. There was no minocycline, chloramphenicol or vancomycin resistant.?S. aureus?was found in this study. SCCmec type III and IIIA were predominant in our study. Our data highlighted that multidrug-resistant strains of MRSA caused severe problems in Shantou, China. However, some of the old agents, such as minocycline, doxycycline and chloramphenicol are highly effective against MRSA isolates.