Development of a loop-mediated isothermal amplification assay for rapid detection of Streptococcus suis serotype 2

摘要

The development of a loop mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of Streptococcus suis, an economically important swine pathogen is reported. Primers were designed targeting conserved region of the capsular polysaccharide (cps2J) gene of S. suis serotype 2 and 1/2. The LAMP assay produced reliable amplification in 60 min at isothermal conditions of 60°C. Genomic DNA extracted from S. suis serotype 2 and other related bacterial species causing illness in swine were used to optimize and validate the sensitivity and specificity of the assay. Based on a serially diluted genomic DNA of S. suis, the LAMP assay was found to be 100-fold more sensitive than the currently used conventional polymerase chain reaction for S. suis. These results indicate that LAMP can be an alternate method for the rapid and sensitive detection of S. suis, especially in resource-poor countries.