Detection of deep fungal infection in SLE patients with multiplex fluorescent real-time PCR

摘要

To establish a rapid, sensitive and specific detection method for?deep fungal infection, we detected the deep infection of?Candida albicans?and?Aspergillus flavusin patients with systemic lupus erythematosus by using multiplex fluorescent real-time PCR (MFRT-PCR). Firstly, the positive rate, sensitivity and specificity of multiplex fluorescent quantitative PCR for detecting the fungal infection was tested in 20 specimens from systemic lupus erythematosus patients with?C. albicans?and?A. flavus?infections. Then, 20 specimens from SLE patients with suspected deep fungal infections and 20 other microorganism infections were detected with MFRT-PCR. Results showed the positivity rate and specificity of both 100% for detecting the deep?C. albicans?and?A. flavus?infection. Its detecting sensitivity for deep fungal infection was 75%, which was significantly higher than that of fugal culture method (40%) (P < 0.05). MFRT-PCR is a better method for detecting deep?C. albicans?andA. flavus?infections, with higher sensitivity and specificity than fungal culture.