Survey frequency of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli and Klebsiella pneumoniae strains isolated from urinary tract infection in Iran

摘要

Extended-spectrum beta-lactamases (ESBLs) are defined as β-lactamase capable of hydrolyzing third generation cephalosporin's and inhibited by β-lactamase inhibitor. Urinary tract infection (UTI) is a one of the most prevalent infection in worldwide and is the second most common infection. Most of UTI are due to?Escherichia coli.?Antibiotic resistance in ESBLs strains is one of the emerging health related problem in the world nowadays. The present study was performed at Tow Tertiary Care Hospitals in Isfahan, Iran during a 14 month period (7th June, 2008 to 6th July, 2010). Standard microbiological methods were performed. In order to validate the ESBLs producing strains, the ceftazidim, ceftazidim clavulanic acid, cefotaxim, cefotaxim clavulanic acid (according to CLSI, 2010) were used by disk diffusion method. Results from 91 samples showed that the frequency of?E. coliand?Klebsiella pneumoniae?strains was 84/6 and 15/4% respectively, while the Frequency of ESBLs in?E. coli?and?K. pneumoniae?strains was obtained in 27 samples as 35/06% and 5 samples as 35/71%), respectively. According to result, there is high prevalence of ESBLs in?E. coli?and?K. pneumoniae?because the third generation cephalosporins are usually in the first line against too many severe infections. Justifiable use of this method will be an effective means of controlling and decreasing spread of ESBLs strains.