Histopathological Examination of the Liver and Gills of Clarias gariepinus Treated With Glyphosate

摘要

Healthy live fishes Clarias gariepinus (average weight and length; 40-50 g and 12-22 cm) were procured from a farm and acclamatized at 28±1.2°C for a minimum of 2 weeks prior to treatment. The water was changed once every 48 h to maintain the test medium. About 10 fishes each in Groups A-D with two replicates were exposed to 0.02, 0.04, 0.08 and 0.00% v/v of glyphosate in that order for a period of 15 days. Fishes were killed after the 5th, 10th and 15th days their gills and liver removed and prepared for histhopathological observation. They were fixed in bouin?s fluid for 24 h, washed with 70% ethanol and dehydrated through a graded series of ethanol. They were embedded in paraffin, sectioned at 4-5 μm thickness stained with haematoxylin and eosin and examined using light microscope and photomicrography. The gills of both the control and treated fishes from histopathological examination showed no damage. However, the liver of treated fishes showed different degrees of damaged ranging from mild portal to congestion of central veins.