La Dramaticaly Enhances the Accumulation of Tanshinones in Salvia Miltiorrhiza Hairy Root Cultures

摘要

The accumulation of tanshinones (tanshinone I, tanshinone II and cryptotanshinone) and the expression of key enzymes (HMGR?HMGS?KSL?DXR?CMK) of tanshinones biosynthetic pathways in Salvia miltiorrhiza hairy root cultures induced by La elicitor were investigated in this paper. La at 0.01mmol·L -1 and 0.1mmol·L -1 obviously stimulated the expression of HMGR on day 1 after treatment. The activity of HMGS and KSL was enhanced by La treatment at the earlier stage of the elicitation treatment and declined gradually at later stage. The activity of CMK was significantly improved with the treatment of La at dilution of 0.01mmol·L -1 on day 1 after treatment. La at 0.01mmol·L -1 and 0.1mmol·L -1 improved the activity of DXR by 161.8% and 378.4% respectively on day 3 after treatment. The content of tanshinone I was markedly enhanced by La at 0.01mmol·L -1 and 0.1mmol·L -1 on day 1 after treatment. La at 0.01mmol·L -1 enhanced the content of tanshinone IIA, which was estimated to be 40.9% higher than the control. The content of cryptotanshinone was significantly improved by 92.4% and 71.5% respectively on day 1 and 3 after treatment with La at 0.01mmol·L -1 , while it was reduced by La at 0.01mmol·L -1 , 0.1mmol·L -1 and 1mmol·L -1 7 days after treatment. In summary the accumulation of tanshinones and expression of key enzymes of tanshinones biosynthetic pathways were improved by La elicitor with lower dose while inhibited with higher dose. And the effect of La on the accumulation of tanshinones lagged behind its effect on the expression of key enzyme gene.