The role of membrane aquaporin-1 (APQ-1) in the photobiomodulation (PBM) on erythrocyte deformability will be studied in this paper with human dehydrated erythrocytes as echinocytic shape alterations lead to decreased cellular deformability. Human dehydrated erythrocytes were irradiated with low intensity He-Ne laser irradiation (LHNL) at 0.9, 1.8, 2.7, and 4.4 mW/cm2for 5, 15, and 30 min, respectively, and APQ-1 inhibitor, 0.2 μmol/L HgCl2, was used to study the role of APQ-1 in mediating PBM with LHNL at 4.4 mW/cm2for 5 min. Comprehensive morphological parameters of an intact cell such as contact area, perimeter, roundness and erythrocyte elongation index (EEI) were measured to characterize erythrocyte deformability with fast micro multi-channel spectrophotometer. It was observed that the dosage of LHNL improvement of the morphological parameters of dehydrated erythrocytes was morphological-parameter-dependent, but the Bunsen-Roscoe rule did not hold for roundness. The LHNL at 4.4 mW/cm2for 5 min significantly improved the contact area (P<0.05) and EEI (P<0.05) of the dehydrated erythrocytes, but the improvement was significantly inhibited by 0.2 μmol/L HgCl2(P<0.05). It was concluded that AQP-1 might mediate the effects of LHNL on erythrocyte deformability, which supports the membranotropic mechanism of PBM.
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机译：本文将研究人类脱水红细胞中膜水通道蛋白1（APQ-1）在光生物调节（PBM）对红细胞变形性中的作用，因为棘皮细胞形状改变会导致细胞变形能力降低。分别以0.9、1.8、2.7和4.4μmW/ cm2的低强度He-Ne激光辐照（LHNL）分别照射人脱水的红细胞5、15和30μmin，APQ-1抑制剂0.2μμmol/ L HgCl2，用来研究APQ-1在以4.4μmW/ cm2的速率与LHNL介导5分钟的PBM中的作用。用快速微多通道分光光度计测量完整细胞的综合形态参数，例如接触面积，周长，圆度和红细胞伸长指数（EEI），以表征红细胞的可变形性。可以观察到，LHNL改善脱水红细胞形态参数的剂量与形态参数有关，但本生-罗斯科规则并不适用于圆度。 LHNL在4.4μmW/ cm2下持续5μmin可以显着改善脱水红细胞的接触面积（P <0.05）和EEI（P <0.05），但是0.2μμmol/ L HgCl2可以显着抑制这种改善（P <0.05）。结论是AQP-1可能介导LHNL对红细胞变形性的影响，这支持PBM的跨膜机制。
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