The role of membrane aquaporin-1 (APQ-1) in the photobiomodulation (PBM) on erythrocyte deformability will be studied in this paper with human dehydrated erythrocytes as echinocytic shape alterations lead to decreased cellular deformability. Human dehydrated erythrocytes were irradiated with low intensity He-Ne laser irradiation (LHNL) at 0.9, 1.8, 2.7, and 4.4 mW/cm2 for 5, 15, and 30 min, respectively, and APQ-1 inhibitor, 0.2 μmol/L HgCl2, was used to study the role of APQ-1 in mediating PBM with LHNL at 4.4 mW/cm2 for 5 min. Comprehensive morphological parameters of an intact cell such as contact area, perimeter, roundness and erythrocyte elongation index (EEI) were measured to characterize erythrocyte deformability with fast micro multi-channel spectrophotometer. It was observed that the dosage of LHNL improvement of the morphological parameters of dehydrated erythrocytes was morphological-parameter-dependent, but the Bunsen-Roscoe rule did not hold for roundness. The LHNL at 4.4 mW/cm2 for 5 min significantly improved the contact area (P<0.05) and EEI (P<0.05) of the dehydrated erythrocytes, but the improvement was significantly inhibited by 0.2 μmol/L HgCl2 (P<0.05). It was concluded that AQP-1 might mediate the effects of LHNL on erythrocyte deformability, which supports the membranotropic mechanism of PBM.
展开▼
机译:在本文中,将膜水疗法-1(APQ-1)在光生成的红细胞变形性上的作用将用人脱水红细胞研究,因为棘爪形状改变导致细胞易变形性降低。用低强度He-Ne激光照射(LHN1)在0.9,1.8,2.7和4.4mW / cm 2的低强度He-Ne激光照射(LHN1),分别为5,15和30分钟,以及APQ-1抑制剂,0.2μmol/ L HgCl2,用低强度He-Ne激光照射(LHN1)照射。 ,用于研究APQ-1在4.4mW / cm 2的LHN1中介导PBM的作用5分钟。测量完整细胞如接触面积,周长,圆度和红细胞伸长指数(EEI)的综合形态学参数,以表征红细胞变形性与快速微通道分光光度计。观察到脱水红细胞形态参数的LHNL改善的剂量是形态学 - 参数依赖性,但Bunsen-Roscoe规则没有持圆形。 4.4mW / cm 2的LHN1 5分钟显着改善了脱水红细胞的接触面积(P <0.05)和EEI(P <0.05),但是改善0.2μmol/ L HgCl2(P <0.05)。得出结论是,AQP-1可以介导LHN1对红细胞变形性的影响,这支持PBM的膜激机制。
展开▼