ANTIMICROBIAL ACTIVITY OF OLIGOMERIC β–DEFENSIN ISOLATED FROM HEART TISSUE OF CAPRA HIRCUS

摘要

Objective: The aim of the present study was to isolate an antimicrobial peptide from heart tissue of Capra hircus (Goat) and to check its antibacterial activity against antibiotic resistant and antibiotic sensitive bacterial strains. Methods: Total protein was extracted from heart tissue of goat using extraction buffer, purified by cation exchange column chromatography and tested in vitro for antibacterial activity against MTCC bacterial strains as well as clinical isolates using the agar well diffusion method. MIC was also checked by microbroth dilution method. Mass spectrometry was performed for partially purified protein fraction. Results: The present work revealed that crude protein extracted from the heart tissue of goat was found to have antimicrobial activity. Partially purified protein fraction also showed the zone of inhibition and MIC value against all the bacteria tested. Mass spectrometry data showed the molecular weight of the peptide to be ~6.5 kD along with peptide to be present in the oligomeric form. Conclusion: The present study showed that the defens in is an antimicrobial peptide with broad range of effect which is present in dimer, trimer and oligomeric form. Keywords: Antimicrobial peptide (AMPs), Capra hircus (Goat), Defensin, Mass spectrometry, Oligomerization.