The Morphometrical Characterisation of Normal Blood Cells of Two air-breathing fishes, Clarias batrachus and Anabas testudineus

摘要

Morphometrical analysis of blood cells plays a major role in fish pathology. Study of blood cells in many different species of fishes provides an interesting comparison of cell size in relation to their activities and habits. For morphometrical analysis of blood cells blood was drawn from the caudal vein and blood smears were prepared immediately after blood was obtained and stained with Giemsa stain. Blood cells and its nucleus length and breadth were measured in micron meter. In this investigation five types of blood cells were found those are erythrocyte, lymphocyte, monocyte, eosinophil and neutrophil but no basophil was observed in Anabas testudineus as well s in Clarias batrachus. Nucleus length and breadth could not measured in granulocytes because of lobed nucleus. Erythrocyte cell length and breadth shows highly significant difference (p0.001) with respect to sex in C .batrachus and A.testudineus and also between these two different fishes. Erythrocyte nucleus length and breadth shows significant sexual difference (p0.001) between different fishes taken for this study. C.batrachus only shows significant sexual difference in nucleus length and breadth which is absent in A.testudineus. Lymphocyte cell length do not differ significantly but cell breadth shows significant sexual dimorphism at p0.05 only in A.testudineus. Lymphocyte nucleus length and breadth shows highly significant difference (p0.001) between these two different air breathing fishes taken for this study but does not show sex wise difference. Among the granulocytes eosinophil and neutrophil do not differ significantly sex wise as well as between these two species. Monocyte cell length (p0.05) and breadth (p0.01) differ significantly between these two species of fish but does not show sex wise difference.