THE BASIC GENE DESIGN PROCESS FOR EXPRESSION OF HETEROLOGOUS PROTEINS IN TRANSGENIC PLANTS

摘要

Transgenic plants are one of the promising expression systems for the production of heterologous proteins, especially therapeutic proteins and peptides. The other expression systems are bacteria, mammalian cell culture, transgenic animals, yeast and fungi. Transgenic plants as bioreactors for the production of useful proteins based pharmaceuticals is not very popular, primarily because of low expression of transgene in plants. With recent development of some gene design strategies using different softwares for best codon usage, study of codon pairs, extreme GC content, eliminating repetitive sequences, avoiding unfavourable mRNA secondary structures and study of restriction sites, it is possible to design a best gene sequence expressing highly in the transgenic plants. The aim of the present study is to understand the factors affecting heterologous protein expression in transgenic plants and to develop a basic gene design process using different computer programs and softwares available in literature leading to high level expression of therapeutic proteins and peptides in plants