SIMPLE AND SELECTIVE SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF MOSAPRIDE CITRATE BY DIAZO COUPLING REACTION IN PHARMACEUTICAL FORMULATIONS

摘要

Three simple and selective spectrophotometric methods have been developed for determination of mo sapride citrate (MDC) in pharmaceutical formulations. The proposed methods are based on the diazotization of MDC with sodium nitrite and hydroc hloric ac id, followed by coupling with resorcinol, 1-benzoylacetone and 8-hydroxyquinoline in alkaline medium for metho ds A, B and C respectively. The colored azo dyes were quantitated photometrically at 520, 428, and 535 nm fo r methods A, B and C, respectively. Different variables affecting the reactions are optimized. Beer's law is obeyed over the concentration ranges of 1.0-24.0, 1.0-28.0 and 1.0-20.0 μg.mL-1 for methods A, B, and C, respectively. The results of the proposed metho ds are validated statistically. The proposed methods were utilized for estimation of MDC in pharmaceutical do sage forms with good accuracy and precision. The results obtained by the proposed methods were compared favorably with those of the reference metho d