DEVELOPMENT AND VALIDATION OF UV-SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF PARACETAMOL AND DOMPERIDONE IN BULK AND TABLET DOSAGE FORM

摘要

Two new simple, accurate and precise spectrophotometric methods have been developed for simultaneous determination of Paracetamol and Domperidone in a binary mixture. In Method I absorbance were measured at 243.4 nm and 284.12 nm corresponding to the absorbance maxima of Paracetamol and Domperidone. In Method II, two wavelengths 284.12 nm, the λmax of Domperidone and 270 nm, an iso‐absorptive point were selected. Linearity range was observed in the concentration range of 15‐30 μg/ml for Domperidone and 11‐16μg/ml for Paracetamol. Concentration of each drug was obtained by using the absorptivity values calculated for both drugs at wavelengths, 243.4 nm, 284.12 nm and 270 nm. Developed method were applied to marketed formulation. The methods were validated statistically and recovery study was performed to confirm the accuracy of both methods. The methods were found to be rapid, simple, accurate and precise