Invitro Cytotoxic Activity of Marsilea Quadrifolia Linn of MCF-7 Cells of Human Breast Cancer

摘要

The aim of the present study was to evaluate the invitro cytotoxic activity of methanol, aqueous and ethyl acetate extracts of leaves of Marsilea quadrifolia on MCF-7 cells from human breast cancer . Qualitative phytochemical screening tests were performed to detect phytochemicals in the extracts. Antioxidant activity of the plant extract was then characterised using , Diphenyl_β- picryl hydrazyl (DPPH) radical scavenging method. Antioxidant activity using DPPH was found to increase, in concentration dependent manner. All the three extracts ethylacetate, aqueous and methanol exhibited potential antioxidant activity with an IC50 value of 10, 125 and 25-g/ml respectively, when compared to the standard BHT with an IC50 value of the 7.5 -g/ml. The cytotoxic activity of the extracts of Marsilea quadrifolia on MCF-7 cells from human breast cancer was investigated invitro 3-(4) 5-Dimethyl-thiazol-zyl)- 2,5 biphenyl tetrazolium bromide ( MTT). The results showed decreased cell viability and cell growth inhibition in dose dependent manner. The IC50 value of standard 5 fluorouracil, methanol,ethylacetate and aqueous extracts were 9.3, 39.06, 47.82 and 187.5-g/ml respectively. Methanol and ethyl acetate extracts of M.quadrifolia demonstrated strongest antioxidant and anti-proliferative activities. The findings from this study indicated that methanol and ethylacetate extracts of M.quadrifolia leaf possessed vast potential as medicinal drug especially in breast cancer treatment. .