STUDY ON ANTIMICROBIAL ACTIVITY OF NOCARDIA sp. STRAIN TP1 ISOLATED FROM TANGKUBAN PERAHU SOIL, WEST JAVA, INDONESIA

摘要

Objective: The aim of this research was to investigate the growth profile of Nocardia sp. strain TP1, as well as antimicrobial acitvity of Nocardia sp. strain TP1 fermentation broth and extract. Methods: Antimicrobial compound was produced by liquid fermentation method. Microbe used in this study was identified as Nocardia sp. strain TP1. Nocardia sp. strain TP1 was isolated from soil of Tangkuban Perahu volc ano region, West Java Indonesia. This isolate was grown in several media composition composed of glucose, starch, tryptone, yeast extract, and calcium carbonate using 7 L fermentor. The fermentation broth was extracted by liquid-liquid extraction using n-butanol, ethyl acetate and chloroform solvents at pH 4.5, 7 and 9. Then, the active substance was detected by bioautography using ethyl acetate-toluene (8:2) as eluent. Results: The highest activity of the culture against methicillin sensitive Staphylococcus aureus (MSSA) was shown after 36 and 38 hours fermentation and 36 hours after fermentation against M. gypseum. Antimicrobial activity of n-butanol extract at pH 7 was shown by MIC value of 128 ppm and 64 ppm against MSSA and M. gypseum, respectively. Bioautography examination on MSSA and M. gypseum showed antimicrobial activity was produced by active spot which gave Rf value of 0.85. Conclusion: Secondary metabolite was produced by Nocardia sp. strain TP1 in fermentation broth active against MSSA and M. gypseum