ANTIDIABETIC ACTIVITY AND ANTI-OXIDANT ACTIVITY OF NIDDWIN, A POLYHERBAL FORMULATION IN ALLOXAN INDUCED DIABETIC RATS

摘要

Objective: The present study was focused to evaluate the antidiabetic activity and antioxidant activity of NIDDWI N, a polyherbal formulation in alloxan induced diabetic rats. Methods: Alloxan induced diabetic rats were divided into four groups of five each. Group-I was given aqueous suspension of 2% gum acacia, Group-II and III was given aqueous suspension of NIDDWIN 50mg/kg and 100mg/kg, Group-IV was given aqueous suspension of Glibenclamide 10mg/kg were given orally for 10days. The blood samples were collected before and after administration of drugs at 0, 2, 4, 6, and 8hrs on 1st, 5th, and 10thday from retro-orbital sinus, serum was separated and estimated for glucose, cholesterol and triglycerides levels. On 10thday pancreas were isolated from all the groups and subjected for histopathological studies. Diabetic rats were evaluated for anti-oxidant activity by using NIDDWIN. Results: NIDDWIN showed significant antidiabetic activity at 4thhr on 1st, 5thand 10thday was found to be effect in comparable with standard Glibenclamide 10mg/kg. Histopathological results of NIDDWIN showed positive results when compared with standard Glibenclamide 10mg/kg. NIDDWIN significantly showed the percentage reduction of lipid per-oxidation levels in diabetic rats. Conclusion: NIDDWIN a polyherbal formulation concluded that it possesses antidiabetic activity and anti-oxidant activity in diabetic rats and further NIDDWIN should be evaluated to develop probable mechanism of action