Potential Role of Curcumin and Garlic Acid against Diazinon and Propoxur Hepatotoxicity

摘要

Diazinon, an organophosphorus insecticide and propoxur, a carbamate insecticide have been used in agriculture and public health for several years. The aim of the present study was to evaluate the oxidative stress caused by the two insecticides and biochemical changes in adult male Wistar rats and to evaluate the protective effect of curcumin and garlic. Diazinon (10 mg/kg per day in corn oil), propoxur (10 mg/kg per day in corn oil) alone or with curcumin (100 mg/kg per day in corn oil) and/or garlic (20 mg/kg per day in distilled water) were given to rats (n=11) orally through gavage for four weeks. Biochemical parameters in serum [total protein, albumin, triglyceride (TG), cholesterol, uric acid, urea, creatinine, γ-glutamyl transpeptidase (γ-GT), glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH), catalase (CAT) and total antioxidant capacity (TAC)], superoxide dismutase (SOD) and glutathion reduced (GSH) were determined in liver homogenate and malondialdehyde (MAD) was determined in RBCs. All ultrastructural changes were investigated at the end of 4th week. Results obtained showed that albumin, total protein, GOT, GPT, LDH, creatinine, urea, GSH and SOD were statistically high significance (p<0.001), γ-GT, uric acid, CAT, TAC and cholesterol were statistically significance (p<0.01) when diazinon or propoxur-treated groups compared to control group. On the other hand, GOT, GPT, LDH, GSH and SOD were statistically high significance (p<0.001), albumin, total protein, γ-GT, urea, CAT and TAC were statistically significance (p<0.01) when curcumin and/or garlic + diazinon or propoxur-treated groups compared to diazinon or propoxur-treated groups. We conclude that curcumin and garlic decreases diazinon and propoxur oxidative toxic effects and hepatotoxicity.