Study of Multiple Sequence Alignment and Phylogenetic Analysis of CFA8 Protein Causing Hemophilia A

摘要

Hemophilia A, an X-linked disorder that occurs due to deficiency of clotting factor VIII (CFA8). It is the most common type of Hemophilia. CFA8 is located at chromosome Xq28. Due to deficiency of CFA8 the Factor XI is not released, hence, no blood clotting is occurring. Deficiency of CFA8 occurs due to mutation (missense, nonsense) in CFA8. MODELLER program was used to build the comparative 3D homology model of CFA8 (target) based on 1KCW (Ceruloplasmin_Homo sapiens). The Motifs are found from the result of MSA through ClustalX. Through MSA result, 20 different Motifs were observed in the families selected for carrying out the MSA with 43% homology between the sequences of CFA8 and 1KCW. The Phylip program generated the tree show that CFA8 and 1KCW has long divergence between them. The divergence shows the mutation in the sequences.