Preliminary Phytochemical Screening and Heavy Metal Analysis of Leaf Extracts of Hippophae rhamnoides Linn”

摘要

Chilli plants infected with leaf curl virus (geminivirus) are characterized by vein clearing, upward curling, deformation of leaves stunting of plants and abscission of flower buds. The virus was identified as chilli leaf curl virus (CLCV) on the basis of mechanical inoculation on Nicotiana tabacum and Chenopodium quinoa and by PCR. MS medium amended with BAP (3.0mg/l) and IAA (3.0mg/l) was used for shoot proliferation. A maximum of 34.26±0.06 number of shoots was obtained. Elongated shoots were rooted on 1/2 MS supplemented with IBA (2.0 mg/l) and 2% activated charcoal. Regenerated plants gave negative results for CLCV by PCR with specific primers. Virus free chilli plants (80%) were obtained from optimum size (0.5 mm) of meristem tips. Virus indexing by PCR was found to be a reliable method for confirmation of CLC virus free nature of the regenerated plantlets. This is the first report of geminivirus-free chilli plants production through meristem tip culture.