PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF LAWSONIA INERMIS LINN (HENNA) LEAF EXTRACTS AGAINST REFERENCE BACTERIAL STRAINS AND CLINICALLY IMPORTANT AMPC ΒETA-LACTAMASES PRODUCING PROTEUS MIRABILIS

摘要

Phytochemical screening and antibacterial activity o f aqueous, ethanol, methanol, ethyl acetate and chloroform extracts of Lawsonia inermis Linn leaves were tested against reference bacterial strains (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeuroginosa, Proteus mirabilis, Salmonella typhi, Vibrio cholerae, Staphylococcus aureus, Methicillin Resistant Staphylococcus aureus) and clinical isolates (Staphylococcus aureus and AmpC β-lactamases producing Proteus mirabilis). The solvent extracts of L. inermis leaves exhibited profound antibacterial activity against the bacterial pathogens tested. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts for Gram positive species were between 34-64 μg and 39-74 μg . MIC and MBC of the extracts for Gram negative species were between 46-71 μg and 51-75 μg. The role of the plant material was apparent in inhibiting the growth of clinically important AmpC β-lactamases producing Proteus mirabilis, which have developed resistance to commonly prescribed antibiotics used for treating infections caused by uropathogens. The findings of the present study may emphasize the use of L. inermis leaf extracts with antibiotics for restricting the growth of clinically important multi-drug resistant uropathogens