A Study on the Type II Antidiabetic Activity of Methanolic Extract of Marine Algae, Gracilaria edulis and Sargassum polycystum

摘要

To evaluate the Antidiabetic activity of methanolic extract of marine algae Sargassum polycystum (SP) and Gracilaria edulis (GE) in fructose induced type II diabetic mellitus. The marine algae Sargassum polycystum and Gracilaria edulis were dried under shade and then powdered, and extracted with methanol. Preliminary phytochemical studies and acute toxicity studies were also carried out on methanolic extract of SP, and GE. For type II Diabetes was induced in Male rats (200-250g). Animals were fed with a high-fat, high- sugar diet (normal diet mixed with 66% (w/v) fructose, 10% coconut oil, and 20% protein). The high fructose diet consisting 10% (w/v) of fructose concentration was delivered ad libitum through drinking water for 10 weeks. Standard received Metformin (300mg/kg). During the study, body weight and fasting blood glucose level were taken at 0 and 30th day. At the end of study, animals in all groups were sacrificed, blood sample, pancreas were collected. Biochemical parameters such as total cholesterol, HDL cholesterol, TG, LDL cholesterol, serum insulin, histopathological studies of pancreas were performed. Oral administration of methanolic extract of SP, and GE (250 mg/kg & 500mg/kg) showed antidiabetic activity in fructose induced diabetic animals. This was evidenced by recovery of body weight, reduction in fasting serum glucose, hypolipidaemic effect. It can be promising in the management of complications and severity of the metabolic abnormality of type ll diabetes.