CYTOTOXIC EFFECT OF INK EXTRACTS OF CUTTLEFISH AND SQUID ON CHICK EMBRYO FIBROBLASTS

摘要

: The aim of the present study is to evaluate the cytotoxic effect of the ink extracts of different specis of cuttlefish (Sepia pharaonis female, Sepia pharaonis male, Sepiella inermis female, Sepia aculeata female) and squid (Loligo duvauceli female). The ink extracts were prepared by delipidation with acetone and extracted by Tris HCl buffer. The cytotoxic effect of the drug was studied using Trypan blue cell viability assay andethidium bromide /acridine orangestaining. The ink extracts from different species of squid and cuttlefish inhibited cell proliferation and induced cell death in a dose dependent manner. The cytotoxic effects induced by cuttlefish ink extract on chick embryo fibroblast cells involve typical apoptotic changes like nuclear condensation, studied using ethidium bromide and acridine orange staining. The ink extracts from the female Cuttlefish, Sepia pharaonis (25 and 75 μg/ml) showed the highest antiproliferative activity. The results suggested the potent antiproliferative effect of the ink of cuttlefish, Sepia pharaonis on chicken embryo fibroblasts and thereby the therapeutic potential of the ink as an anticancer agent.