IN-VITRO EVALUATION OF THE ANTIOXIDANT POTENTIAL, TOTAL PHENOLIC AND FLAVONOID CONTENTS AND ANTIBACTERIAL ACTIVITY OF LAMIUM ALBUM EXTRACTS

摘要

emLamium album /emis used as a blood purifier, in the treatment of the respiratory tract diseases, diarrhea and bleeding. In this study antioxidants and anti-bacterial activities of its aerial parts and roots were investigated. Extracts were prepared by Soxhlet apparatus. Methanol used as extracting solvent. Phenol and flavonoids contents and antioxidant properties were evaluated by different methods. Different concentrations of extracts were used for determining the MIC. Microorganisms suspensions were prepared in Mueller Hinton broth with different concentrations of extracts and incubated for 24 h at 37 °C, the turbidity of the tubes were observed. MIC and MBC were determined. Total phenolic content of aerial and roots extracts were 242.75 ± 10.13 and 135.0 ± 8.15 and total flavonoid contents were 79.83 ± 4.22 and 30.33 ± 1.08 QE respectively. The DPPH radical-scavenging ability of extracts were 238.4 and 257.0 μg/ml respectively. Reducing power of both extracts increased by increasing the concentrations. The amount of scavenging nitric oxide at the 1600 mg/ml, was 58 and 68%, respectively. ICsub50/sub for chelating activities of extracts were 1139 and 1323 μg/ml for aerial and root extracts, respectively. Antimicrobial property of the aerial parts against emE. coli/em in microdilution method was better than root extract. Its mean diameter of inhibition was 17 mm. On emKlebsiella/em the activity on the root extract was better. Its inhibition diameter was 11.66 mm. In conclusion, the anti-microbial and antioxidant activities of emL. album/em aerial parts was higher than root extract.