Separation and Simultaneous Quantitation of Meclizine Hydrochloride and Pyridoxine Hydrochloride in their Solid and Semi-Solid Preparations using Validated HPLC method”

摘要

The members of Lamiaceae family include aromatic plants that are being used in traditional medicine for various disorders. The therapeutic application of these plants is attributed to the presence of secondary metabolites or phytochemicals such as alkaloids, saponins, flavonoids, glycosides and phenols. This study is a preliminary screening of phytochemicals of Leucas linifolia, Coleus aromaticus, Pogestemon patchouli, which belong to Lamiaceae family. Methanol, ethanol and chloroform extracts of each plant were subjected to both qualitative and quantitative phytochemical screening. The alkaloids, saponins, total phenols, tannins, flavonoids, steroids and proteins were quantified in the extracts by standard spectrophotometric methods. The results of the qualitative analysis of the study plants, revealed the presence of steroids and absence of terpenoids, amino acids in all the leaf extracts. In addition, methanol extracts were positive for alkaloids, saponins, tannins and phenols, flavonoids, carbohydrates and glycosides. Whereas, the ethanol extracts showed fewer constituents in different combination in all the three study plants. However, chloroform is a good solvent for extraction of steroids from these plants since only steroids were present in chloroform extract. The concentration of total phenols and tannins was higher in methanol extract. Pogestemon patchouli had the highest concentration of flavonoids more so in ethanol than either methanol or chloroform extracts. Although, protein concentrations was higher in the ethanol extracts of Leucas linifolia and Coleus aromaticus, methanol extract of Pogestemon patchouli had more protein than ethanol extract. However, chloroform which extracted steroids, is not a good choice for extraction of proteins from these plants. In conclusion, for further studies on the phytochemical and biological structure-function relationship, the use of appropriate solvent for extraction and purification of the specific phytochemical from plants is one of the crucial steps.