HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DRUG RELEASE OF LEVODOPA AND CARBIDOPA IN ENTACAPONE, LEVODOPA AND CARBIDOPA TABLETS

摘要

In pharmaceutical industry, researchers aim at catering to the need of robust analytical methods for analysis of generic drug products. The paper deals with method of analysis of pharmaceutical formulation – Entacapone, Levodopa and Carbidopa tablets for the treatment of Parkinson’s disease. The paper presents a simple and efficient HPLC method that has been developed for a multi component drug formulation for estimation of % drug release of levodopa and carbidopa. This HPLC method uses ‘Cosmosil 5PE-MS 150 x 4.6 mm, 5 μ’ HPLC column, combination of phosphate buffer pH 2.5 and methanol as mobile phase in gradient mode with UV detection at 280 nm. The method was validated and found to be precise, robust, accurate, linear (in range 0.020 to 0.40 mg/mL and 0.005 to 0.100 mg/mL of Levodopa and Carbidopa respectively), and specific for blank and placebo solution ensuring suitability of the method for quantitative determination of % drug release of Levodopa and Carbidopa in presence of Entacapone in multi component pharmaceutical formulation.