DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF NIGELLA SATIVA SEED OIL AND GINGER EXTRACT IN THE SAME DOSAGE FORM

摘要

The combination of Nigella sativa seed oil and ginger extract is used as antiulcer agent and to treat abdominal pain, diarrhoea and flatulence. The paper describes validated simultaneous equation method for the simultaneous estimation of Nigella sativa seed oil and Ginger extract in a combined dosage form. Nigella sativa seed oil and Ginger extract were found to have absorption maxima at 256 and 282 nm respectively in 5% SLS solution. Both these drugs obeyed Beer’s law in the concentration range of 100-500μg/mL. The high values of correlation coefficients (r2) indicated good linearity of calibration curve for both the drugs. The results of analysis have been validated statistically and by recovery study the value of standard deviation ranging from 97-99.21 % for Nigella sativa seed oil and 98-101.6 % for ginger extract were indicative of the accuracy and precision of the proposed method. The proposed method was successfully applied for the determination of nigella sativa seed oil and Ginger extract in the pharmaceutical formulation. This method was found to be simple, sensitive, accurate, precise and economical and applicable for the simultaneous determination of Nigella sativa seed oil and Ginger extract in combined dosage form.