DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR SOME AZOLES IN PHARMACEUTICAL PREPARATION

摘要

A new, simple and rapid reversed-phase high-performance liquid chromatography (HPLC) method was developed and validated for the determination of some azoles drug (Ketoconazole, Isoconazole and Miconazole) in pharmaceutical dosage forms in Jordan market. The HPLC separation was achieved on a C_18?BDS column (100?mm?×?4.6?mm) using a mobile phase of acetonitrile and 0.05 M Ammonium Acetate buffer (70:30,?v/v) that adjusted to pH 6 using phosphoric acid at a flow rate of 1?mL?min?1 and using UV absorbance detection at 254?nm. The method was validated for specificity, linearity, precision, accuracy, robustness. The separation was completed within 11.77 minutes and the assay was linear over the concentration range of 50% to 150%?(r2?=?0.9999), the percent recovery for test and reference formulation was (98.14%-101.12%).