Whole Genomic EST-SSR Development Based on High-Throughput Transcript Sequencing in Proso Millet (Panicum miliaceum)

摘要

Proso millet (Panicum miliaceum L.) with short growing period, is highly suited to drought and semi-arid climate, and thus possesses great potential for research. In this study, 8139 Expressed Sequence Tags-simple sequence repeat (EST-SSR) makers were developed from the transcript sequencing of Proso millet cultivar Yanshu 5. Tri-type was the major type, accounting for 83.65% of all the found SSR types, followed by di-type accounting for 11.1%. CCG/CGG was the most ample repeat-motif types in Proso millet. Twenty four primer pairs of these markers were synthesized for empirical validation and 21 primers obtained targeted fragments by PCR amplification. Three of the 21 markers were polymorphic among 20 Chinese Proso millet landraces which suggested that these EST-SSR markers can be used for genetic studies. Markers developed in this work can be used for agronomic trait location, linkage map construction and molecular breeding in Proso millet in the future.