Fatty Acid Composition of Three Diatom Species Skeletonema costatum, Thalassiosira sp. and Chaetoceros gracilis

摘要

The goal of this research work is to compare the lipid content of three diatom species (Skeletonema costatum, Thalassiosira sp., and Chaetoceros gracilis) and to identify the fatty acid composition of those species. Highest content of lipid was found in Chaetoceros gracilis, while the lowest was in Skeletonema costatum. The extracts of different solvents shown lipid content in some extent where chloroform produced higher lipid content compared to hexane. The highest Fatty Acids Methyl Esters (FAME) found in Chaetoceros gracilis was methyl palmitic (C_(16:0)), 33.29 % extraction with chloroform as solvent and methyl palmitoleic (C_(16:1)), 49.42 % extraction with hexane as solvent. The highest Fatty Acids Methyl Esters (FAME) found in Skeletonema costatum was methyl palmitoleic (C_(16:1)), 31.15 % extraction with hexane as solvent and methyl myristic (C_(14:0)), 41.46 % extraction with chloroform as solvent. The highest Fatty Acids Methyl Esters (FAME) found in Thalassiosira sp. was methyl palmitic (C_(16:0)), 34.17 % extraction with chloroform solvent and methyl palmitoleic (C_(16:1)), 44.72 % extraction with hexane solvent.