Analytical Study of Plasmodium Yoelii Yoelii

摘要

Protein sequence of Plasmodium yoelii yoelii "prt_seq No definition line found" was extracted from GenPept database (ACCESSION: EAA21357), used to predict its hydrophobic, atomic and amino acid composition, PEST region, solvent accessibility, molecular mass, theoretical pI and finally catalytic site detail was identified with their three dimensional structure. Analysis was made using several bioinformatics tool. PEST region was indentified using pestfind tool, whose abundant availability indicates intracellular half-lives of less than two hour. Hydrophobicity was checked using [1] to know it's buried or exposed state in terms of solvent accessibility, also used to find its different physical and chemical properties, secondary structure composition using Hidden Markov models, formation of disulphide bond, effect of point mutation prediction using heat map representation and finally catalytic site identification were made. Online Active site prediction was made using web server [2], where given protein sequence was matched with server's library of catalytic site, resulting in twenty six identified region with maximum score of 0.015 with Orotidine "5-monophosphate decarboxylase" and minimum score of 0.004 with "Endo-alpha-sialidase". Molecular weight of retrieved protein sequence was found to be 32049.9 and Theoretical Pi value was 6.08, protein close to given Pi and molecular mass was matched using TagIdent web server [3], which reveal 126466 protein. The Instability Index was computed to be 36.19 which classifies the protein as stable and the Aliphatic Index showing relative value occupied by aliphatic side chain (alanine, valine, isoleucine, and leucine) was 80.77. The three dimensional structure of catalytic site was visualized using Jmol software.