Choosing an Optimal Suture Material for Prehepatic Portal Hypertension Modeling

摘要

If the catheter size, the thickness of a thread, the place for ligation were determined for the partial portal vein ligation (PPVL), the question of the suture material remains open. The aim of the study was to determine the optimal suture material (silk or prolene) for PPVL. The experiment was performed on 25 male adult outbred rats. The PPVL was carried out using the catheter 20G with thread 4-0. Portal pressure was measured before and 15 days after ligation. The measurements were performed by the differential manometer Testo 510 (Germany), which was connected to the catheter 24G, located in the distal part of the superior mesenteric vein. The animals were divided into two groups: the first group (n=12) - portal vein (PV) was ligated with silk, and the second group (n=13) - PV was ligated with prolene. The average values of portal pressure before PPVL in experimental animals were 7,50 ± 0,20 mmHg. All animals survived in the first group. 7 rats in the second group died on the 1-2 day after surgery because of PV thrombosis. There were no significant differences in the average values of portal pressure in the comparison groups after 15 days: the first group (n = 12) - 11,44 ± 0,06 mmHg, the second group (n = 6) - 11, 24 ± 0,04 mm Hg. Despite the same type of portal hypertension model and its equal severity, the use of prolene thread caused a greater propensity to PV thrombosis.