Decreased SARI expression predicts poor prognosis of Chinese patients with non-small cell lung cancer

摘要

emSARI/em is associated with the risk for several cancers, and loss of emSARI/em expression is frequently found in aggressive and metastatic cancer. Limited evidence shows that emSARI/em is a tumor suppressor gene, but the role of emSARI/em in non-small cell lung cancer (emNSCLC/em) has not been previously reported. This study was to investigate the emSARI/em expression profile in surgically resected lung cancer tissues of Chinese patients by immunohistochemistry and evaluate the relationship between emSARI/em expression and prognosis of lung cancer patients. Furthermore, emSARI/em gene was transfected into lung cancer cells (emA549/em), and the growth curve and cell healing of lung cancer cells were determined, aiming to investigate the influence of emSARI/em on the growth and migration of lung cancer cells in vitro. Results showed that 103 of 195 (52.82%) tissues were positive for emSARI/em. When compared with normal tissues, emSARI/em expression significantly reduced in 50.26% of emNSCLC/em tissues. Patients with negative or reduced emSARI/em expression were more likely to have advanced lung cancer and lymph node metastasis. In squamous carcinoma and adenocarcinoma patients, the emSARI/em expression had no relation with the survival time; However in one-on-one analysis emSARI/em expression in tumor cells and adjacent tissues, patients which tumor cells emSARI/em express reduced than adjacent tissues, survival time was significantly shorter than those without reduction in emSARI/em expression (Log Rank test, emp/em = 0.001). After transfection by emSARI/em gene, the proliferation and migration of emA549/em cells were obviously inhibited (emp/em < 0.001). These results demonstrate that decreased emSARI/em expression may predict a poor prognosis in emNSCLC/em patients, and emSARI/em may serve as a prognostic biomarker and potential therapeutic target for lung cancer.