Toxicity Study of Ricinus cummunis Lnn SeedSuspension in Female Wister Albino Rats

摘要

Ricinus communis seed has been proven effective to prevent conception since time immemorial. This research focuses on the toxicity study of the seed suspension in Wister albino rats. Twenty four (24) rats were used for the sub-chronic toxicity study, while 13 mice for the acute toxicity study. The seed suspension of Ricinus communis seed at three graded concentrations (3.80, 7.60 and 11.40 mg/kg body weight) was administered orally; to Groups I, II and III respectively once every day for the period of one month. The liver and kidney functions were determined after the last administration. Serum alanine amino transferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), urea and creatinine were determined. The activity of ALT, AST, ALP and the concentrations of urea and creatinine at 3.80 mg/kg body weight showed no significant difference (p>0.05) compared to the control. However, a significant increase (p<0.05) in these parameters was observed in rats given 7.60 and 11.40 mg/kg body weight. Similarly a significant increase (p<0.05) in lipid profile was observed in rats given 11.40 mg/kg body weight. Acute toxicity revealed the median lethal dose (LD50) of 1587 mg. Histological analysis of the liver and kidneys of the rats after three months revealed no cellular death, necrosis or inflammation. This indicates that consecutive use of the seed at the traditional dose (3.80 mg/kg in rat; equivalent to 3 seed/70Kg in human) for long period of time is neither hepatotoxic nor nephrotoxic. Ricinus communis seed is therefore safe in rats at the concentrations administered.