EFFECT OF MEDIA COMPOSITION AND CULTURE CONDITION ON PULLULANASE ENZYME FROM EXTREMOPHILE BACTERIAL SPECIES

摘要

Pullulanase is of great significant due to its wide area of potential application. Pullulanase is widely used in industries in the saccharification of starch. It converts starch into glucose and maltose which are used in the production of glucose syrup more efficiently. In this present study the enzyme was isolated by the extremophilic strains of Streptococci isolated from extreme conditions. Pullulanase enzyme 98 KDa was successfully produced and purified by the isolated strains. In this process to study enzyme activity the enzyme substrate reaction was performed based on various parameters temperature 50°C, time 72 hours, and pH 6. As it is clearly stated after the detail analysis of all parameters that the pullulanase isolated in the present study is very stable and active at different parameters. The Pullulanase activity of Gel filtration column chromatography of precipitated protein by ammonium sulphate gave rise 130.66 IU pullulanase activity which was greater than the 0-30% (98.0 IU) and 70-90% (130.66 IU). And thus it can be used in various industrial processes where normal mesophilic enzymes cannot be applied.