MOLECULAR CHARACTERIZATION OF MAP30 GENE FROM MOMORDICA CHARANTIA L.

摘要

Natural products especially from plants have been used for the treatment of various diseases. Momordica charantia or bitter melon, grows in the tropical area is popularly consumed as vegetables and has high medicinal values .It is one of the most promising alternative medicines used as anti-HIV, anti-ulcer, anti- inflammatory, antileukemic, anti-microbial, anti-diabetic, and anti-tumor. Proteins like momordin, alpha- and beta- momorcharin and cucurbitacin B of Momordica charantia were also tested for possible anticancerous effects .Chemical analogs of these proteins have been developed, patented, and named MAP30.The MAP30gene was amplified from Momordica charantia leaves by Polymerase Chain Reaction .The resultant product was amplified approximately 861 bp.