The Expressions of TGF-β1 and IL-10 in Cultured Fibroblasts after ALA-IPL Photodynamic Treatment

摘要

Background Topical photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) was originally used for treating superficial skin tumors. The application of PDT to other inflammatory dermatoses like acne vulgaris, psoriasis, granuloma annulare, localized scleroderma and lichen sclerosus has recently been introduced. However, the underlying mechanisms are not well understood. We've previously reported the induction of tumor growth factor (TGF)-β₁ and interleukin (IL)-10 after PDT with ALA and intense pulsed light (IPL) in cultured HaCaT cells. Objective The purpose of this study was to investigate the expressions of TGF-β₁ and IL-10 in cultured fibroblasts after PDT with using ALA and IPL. Methods Cultured fibroblasts were treated with ALA-IPL PDT (1μmol/L of ALA; 0, 4, 8 and 12 J/cm2 of IPL). The expressions of TGF-β₁ and IL-10 were investigated by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay. Results TGF-β₁ mRNA and protein were reduced down to 0.52- and 0.63-fold, respectively, after PDT and the IL-10 protein was increased up to 2.74-fold after PDT. Conclusion The reduction of TGF-β₁ was prominent after PDT and so an antisclerotic effect can be expected after PDT. The induction of IL-10 may contribute to the anti-inflammatory effect, which explains the therapeutic benefit of PDT for inflammatory dermatoses.