Clonagem e avalia???§???￡o da express???￡o g???anica do sbGnRH em machos juvenis e adultos de linguado, Paralichthys orbignyanus

摘要

The objectives of this study were to clone sb Gn RH c DNA, evaluate the m RNA levels in different tissues of flounder, and also evaluate brain sb Gn RH expression in juvenile and adult males. Using RT-PCR the cloning of a 297 nucleotides coding region of sb Gn RH from Brazilian flounder brain was demonstrated for the first time. Expression of sb Gn RH was detected in several peripheral tissues. Brain gene expression in the adult flounder was higher than those found in juvenile. These results suggest that sb Gn RH is involved on the Brazilian flounder puberty.