Avalia???§???￡o de diferentes crioprotetores intra e extracelulares na criopreserva???§???￡o de s???amen de touros

摘要

The experiment was designed as 3 x 2 factorial design, with three internal cryoprotectants, glycerol (GLY), etileneglycol (EG) and dymethilformamide (DMF) and two external, egg yolk (YOLK) and density low lipoproteina (LDL). The motility at thawing for GLY-YOLK (53.9?±1.96) was higher than other treatments (P<0.05). The percentage of cells with normal morphology at thawing was not different between EG-YOLK (68.3?±1.58), EG-LDL (72.2?±2.39) and DMF-YOLK (68.7?±1.67), but they were higher than the others (P<0.05). The evaluation of membrane integrity through fluorescent probes at thawing indicate that GLY-YOLK (34.2?±2.28) and EG-YOLK (30.9?±1.32) were not different (P>0.05), but were higher than the others (P<0.05). The evaluation of membrane integrity through hypoosmotic swelling test (HOST) indicate that DMF-YOLK (13.6?±1.30) and DMF-LDL (9.8?±0.78) were different (P<0.05) and lower than the others (P<0.05). The use of ethylene glycol associated to egg yolk can be a viable alternative to the use of glycerol in bull semen freezing protocols.