ANTIDIABETIC AND CYTOTOXIC ACTIVITIES OF METHANOLIC EXTRACT OF TABERNAEMONTANA DIVARICATA (L.) LEAVES IN ALLOXAN INDUCED MICE

摘要

Tabernaemontana divaricata a common garden plant in tropical countries has been used as a traditional medicine. The present investigation was designed to evaluate the antidiabetic activity of the methanol extract of leaves of T. divaricata on alloxen indueced diabetic mouse model. The extract was given intraperitonially at different doses (300 and 400 mg/kg body weight) and the blood glucose levels were measured at 0, 2, 6, 12 and 16 hours of the study period. The antihyperglycemic effect of the extract was compared with metformin, a standard drug. The extract of 400 mg/kg reduced maximum blood glucose level at 12th hour of the treatment period from 14.19±0.47 to 6.86±0.41 mmol/L. The extract was also subjected to Brine shrimp lethality bioassay. LC50 value of the extract was 27.87 μg/ml. So, the present results showed that T. divaricata has potential (P<0.05) antidiabetic activity and low cytotoxicity as compared to standard drugs.