Auricularia auricular polysaccharide-low molecular weight chitosan polyelectrolyte complex nanoparticles: Preparation and characterization

摘要

Abstract Novel polyelectrolyte complex nanoparticles (AAP/LCS NPs) were prepared in this study and these were produced by mixing negatively charged auricularia auricular polysaccharide (AAP) with positively charged low molecular weight chitosan (LCS) in an aqueous medium. The {AAP} was extracted and purified from auricularia auricular, and then characterized by micrOTOF-Q mass spectrometry, UV/Vis spectrophotometry, moisture analyzer and SEM. The yield, moisture, and total sugar content of the {AAP} were 4.5%, 6.2% and 90.12% (w/w), respectively. The {AAP} sample was water-soluble and exhibited white flocculence. The characteristics of AAP/LCS NPs, such as the particle size, zeta potential, morphology, FT-IR spectra, {DSC} were investigated. The results obtained revealed that the AAP/LCS {NPs} had a spherical shape with a diameter of 223?nm and a smooth surface, and the results of the FT-IR spectra and {DSC} investigations indicated that there was an electrostatic interaction between the two polyelectrolyte polymers. Bovine serum albumin (BSA, pI?=?4.8) and bovine hemoglobin (BHb, pI?=?6.8) were used as model drugs to investigate the loading and release features of the AAP/LCS NPs. The results obtained showed that the AAP/LCS {NPs} had a higher entrapment efficiency (92.6%) for {BHb} than for {BSA} (81.5%). The cumulative release of {BSA} and {BHb} from AAP/LCS {NPs} after 24 h in vitro was 95.4% and 91.9%, respectively. The in vitro release demonstrated that AAP/LCS {NPs} provided a sustained release matrix suitable for the delivery of protein drugs. These studies demonstrate that AAP/LCS {NPs} have a very promising potential as a delivery system for protein drugs.