A Pink pigmented facultative Methylotrophic bacterium was isolated from the phyllosphere region of cotton by leaf imprinting method on Ammonium mineral salt agar supplemented with 0.5% of methanol and cyclohexamide. Their distinctive pink pigmentation is due to carotenoid, which render them to be tolerant to extreme light condition and radiation. The isolate was morphologically and biochemically characterized and identified as a member of the genus Methylobacterium. The parameters such as temperature, pH, and carbon source were optimized for the extraction of pigment from PPFM bacteria. The optimum pH for growth and pigment production was around 7.5 at temperature of 25? C with 0.5% of methanol. The PPFM bacterium was mass multiplied at these optimum conditions, pigment was extracted, and the sample was analyzed by UV- visible Spectrophotometer.
展开▼
