ISOLATION OF TAXOL PRODUCING FUSICOCCUM SPECIES FROM CURRY LEAF AND ITS RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS

摘要

Objective: The potential compound of taxol has been used treatment of cancer in the medical field. This study is focused on the extraction of taxol from Fusicoccum sp. Methods: The pathogenic strain was grown in MID medium for the taxol production and extracted from the Fusicoccum culture filtrate and processed to the ultraviolet (UV)-spectroscopy analysis as well as infrared (IR) spectra analysis was done, and the sample was performed to the high-performance column chromatography and further processed to the random amplified polymorphic DNA (RAPD) analysis of specific primer is PGFO1, PGFO2 are performed. Results: UV-visible spectral analysis showed maximum value for 273 nm. The result of IR-spectrum analysis to find out the functional groups present in sample and characteristic peak to conform the existences of OH group. The presence of taxol was confirmed using column chromatography. An analysis of taxol production was on the external standard of authentic taxol. The column chromatography process shows the amount of taxol production by Fusicoccum spp. 325 μg/L. RAPD analysis indicates the genetic relation among all isolates of fungus was analyzed by two random primers PGF01 and PGF02. Conclusion: The present investigation revealed that the taxol production from biological source was a significant effect, and the presence of taxol amount is analysis in Fusicoccum sp. 325 μg/L. The RAPD analysis of genetic stability of in vitro grown fungus and genotypic with two different primers shows that five fungus species with corresponding primer efficiency was amplified.