Using Randomly Amplified Polymorphic DNA for Evaluating Genetic Relationships Among Artemia Species

摘要

We have applied the technique of random amplification of polymorphic DNA (RAPD) to the analysis of the relationships among four species of brine shrimp: Artemia franciscana, A. urmiana, A. sinica, and A. parthenogenetica . Seventy ten- base synthetic oligonucleotides were chosen from 200 that amplified a total of 751 distinct fragments. DNA polymorphisms were found in all the species examined, the highest percentage of polymorphic bands was shown in parthenogenetica as 28. 76 % . Each species was scored for the presence or absence of every amplification product and the data entered into a binary data matrix. Cluster analysis was then performed to create a dendrogram using UPGMA by the NTSYS program. There are significant differences between bisexual sibling species and parthenogenetic populations. A . parthenogenetica provided 94 specific molecular markers, while bisexual sibling species gave 27 specific molecular markers. A . sinica is a species distinguished from the other Old World bisexual species. A. urmiana played an important role in the evolution of A . sinica .