Study of cell death and stages of leukocytes apoptosis in pulmonary tuberculosis patients with different antimycobacterial treatments

摘要

The aim of the study was to evaluate the condition of cytoplasmic membranes of leukocytes and to study their viability, to determine the varieties and stages of cell death of leukocytes in patients with pulmonary tuberculosis treated by different schemes of antimycobacterial treatment. Materials and methods . The study was performed on 30 patients with pulmonary tuberculosis: the 1 st group – 12 patients treated by standard regimen with 1 st line drugs; the 2 nd group treated by individual regimen with 1 st and 2 nd line drugs. Results . The analysis of cytoplasmic membranes of leukocytes in the blood of patients from the 1 st and the 2 nd group showed that the proportion of live intact cells was 71.05±3.66%, that is lower by 20.75 % from the results of control group. In the 2 nd group, the proportion of alive cells was lower by 12.9% than in the 1 st group (р0.05). In the same time, the proportion of leukocytes on the late of apoptosisecrosis, that have deep damages of cell structure, was more than 10% higher than in the 1 st group (р0.05). Conclusions . A significant difference between the groups of patients in the proportion of absolutely alive leukocytes and cells on the late stage of apoptosisecrosis can be a proof of higher activation of apoptosis process by individual treatment regimen that included 1 st and 2 nd line-drugs, compared with standard 1 st line regimen. The obtained data allow us to recommend the study of influence of different anti-tuberculous drugs and regimens on apoptosis process of immune-competent cells, to evaluate the efficacy of pulmonary tuberculosis treatment.