首页> 外文期刊>Artificial cells, nanomedicine, and biotechnology. >Hsa_circ_0067997 promotes the progression of gastric cancer by inhibition of miR-515-5p and activation of X chromosome-linked inhibitor of apoptosis (XIAP)
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Hsa_circ_0067997 promotes the progression of gastric cancer by inhibition of miR-515-5p and activation of X chromosome-linked inhibitor of apoptosis (XIAP)

机译:Hsa_circ_0067997通过抑制miR-515-5p和激活X染色体连锁的凋亡抑制剂(XIAP)促进胃癌的发展

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Circular RNAs (circRNAs) have been revealed to play vital roles in modulating gene expression and participate in several pathological responses including gastric cancer (GC). However, the larger numbers of the circRNAs in GC progression remain undetermined. In the present study, four GC related circRNAs expression profiles from the Gene Expression Omnibus (GEO) database were enrolled. We identified hsa_ circRNA_00067997 (circ_0067997) as an oncogene in GC. qRT-PCR was used to validate the expression of circ_0067997 in GC tissues and cell lines. The results revealed that circ_0067997 was upregulated in GC, and high circ_0067997 expression was associated with the poor overall survival rate of GC patients. Knockdown of circ_0067997 significantly reduced cell viability, inhibited colony formation, and attenuated invasive ability, whereas overexpression of circ_0067997 exhibited opposed effects. Circ_0067997 was identified to be a sponge for miR-515-5p directly. Moreover, XIAP was demonstrated to be targeted and regulated by miR-515-5p. In conclusion, circ_0067997 was identified to be an oncogene in GC by regulating miR-515-5p/XIAP axis.
机译:环状RNA(circRNA)已被揭示在调节基因表达中起重要作用,并参与包括胃癌(GC)在内的多种病理反应。然而,GC进展中更多的circRNA仍未确定。在本研究中,招募了来自Gene Expression Omnibus(GEO)数据库的四个与GC相关的circRNAs表达谱。我们将hsa_ circRNA_00067997(circ_0067997)确定为GC中的致癌基因。使用qRT-PCR验证circ_0067997在GC组织和细胞系中的表达。结果表明,circ_0067997在GC中被上调,而circ_0067997的高表达与GC患者的整体生存率差有关。敲除circ_0067997可显着降低细胞活力,抑制菌落形成并减弱侵袭能力,而circ_0067997的过表达则表现出相反的作用。 Circ_0067997被鉴定为直接用于miR-515-5p的海绵。此外,XIAP被证明是由miR-515-5p靶向和调控的。总之,通过调节miR-515-5p / XIAP轴,circ_0067997被鉴定为GC中的癌基因。

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